Organ-on-a-Chip Platform

Organ-on-a-Chip Platform

The Fluigent/Micronit organ-on-a-chip platform focuses on modeling the main biochemical and biophysical features of the native environment of cells in order to induce their growth and differentiation as functional tissues. Air-liquid interface, flow induced shear stress, mechanical stimulation, biochemical gradient, cell-cell coculture have been reported to significantly improve the functionality of in vitro models. All these parameters are controlled by the system.

Picture on the right: Skin on chip grown on the membrane. Courtesy of Dr A El Ghalbzouri, LUMC, The Netherlands.

Find a publication
skin-on-chip-membrane-cell-biology

Do you need more information?

Fluigent has partnered with Micronit to develop a versatile fully integrated organ on a chip platform which reproduces numerous characteristics of the in vivo environment of cells. This platform comprises a flow control system connected to a resealable glass chip separated into two flow chambers by a transversal porous membrane.

organ-on-a-chip-platform-fluigent-set-up

 

Benefits:

  • Long term stable cell culture under perfusion (up to 1 month)
  • Air/liquid or liquid/liquid interfaces with distinct monitored flow rates in each chamber
  • Reproduction of flow induced physiological shear stress
  • Cell co-culture in controlled microenvironments
  • Biomechanical stimulation through membrane stretching
  • Biochemical gradient
  • Sample collection from apical and basolateral flow chambers

 

Applications:

  • Microphysiological systems/Organ-on-chip
  • Tissue/ cell stretching
  • Cell culture under perfusion
  • Hyperoxia /hypoxia in cell culture
  • Trans-membrane invasion assay
  • Quantification of molecular uptake and transport
  • Drug discovery/screening

organ-on-chip-hut-on-chip-intestinal-epithetial-cells

Gut-on-chip: coculture of intestinal and endothelial cells on the membrane. (i) Basal view of the membrane, (ii) transversal view of the membrane. (Nuclei: blue, actin: green, tight junctions: red). Picture courtesy of Dr Meike van der Zande, RIKILT – Wageningen University & Research, The Netherlands.

c78851e63dfe4cfcbdee793819c8e5a4''''''''