EMMA
EMMA™ is based on Heteroduplex Analysis (HDA) by multi-capillary electrophoresis with an unprecedented sensitivity, comparable with this of DHPLC or better depending on the application, thanks to a new proprietary matrix composition, combining sieving and chromatographic effects.EMMA™ also diagnoses in the same run, at no extra cost, large scale-rearrangements. EMMA™ saves on the PCR step thanks to size multiplexing.EMMA™ is ideal for the detection of unknown mutations for a diagnostic use as well as for new mutations discovery.
The first product in our line, EMMA™, is designed for the detection and discovery of unknown mutations.Enhanced Mismatch Mutation Analysis (EMMA™) is a mutation detection method alternative to sequencing. It combines all the advantages of screening before sequencing strategy (High throughput, great reduction of sequencing costs, high productivity).
EMMA™ offers:
• Sensitivity at least equal to dHPLC
• Easy differenciation polymorphism vs. variant• High Throughput (covering up to 1 400 fragments/day with a 16 capillaries sequencer)
• High PCR throughput due to size multiplexing (384 fragments amplified on a 96 plate PCR)
• Simultaneous dection of large scale rearrangements and point mutations• Ease of use: - No prior optimization required: a unique set up condition is used whatever the fragment - Not sensitive to GC content: The unique set up condition can be used even for GC rich fragments and for fragments with a GC rich region flanked by two AT rich regions. - Easy interpretation thanks to a time-saving dedicated analysis software Emmalys.• Increase of mutation detection productivity without affecting your expenses.
Fluigent is developing original and proprietary matrices for DNA separation by capillary and multicapillary electrophoresis. Compatible with existing commercial instruments, these matrices exist in different versions, for either dsDNA or ssDNA separations. They combine outstanding resolution with dynamic coating capabilities and long capillary lifetime.