- Easy to install and use
- Many automation possibilities
- Fast and...
The use of water-in-oil droplets in microfluidics in high-throughput screening is rapidly gaining acceptance. The main application areas currently involve screening cells as well as genetic material for various mutations or activity. Here the aim is to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression.
There were several steps to the study:
The second emulsion was generated encapsulating an aqueous-phase IVT (In Vitro Transmission) mixture in the same organic phase.
For these studies, it is very important that the droplets are generated at the correct frequency, and at uniform size. That’s where the use of the MFCS™-EZ is critical with the generation of very stable flows and the accurate control of the different phases.
A graphic representation of the droplet chip used to combine the PCR and IVT droplets is below.